کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5974445 1576212 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HIV-1 gp120 induces autophagy in cardiomyocytes via the NMDA receptor
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
HIV-1 gp120 induces autophagy in cardiomyocytes via the NMDA receptor
چکیده انگلیسی

BackgroundHIV-1 envelope glycoprotein gp120 (gp120) is considered as one of the major virulent proteins responsible for the involvement of the cardiovascular system. Autophagy as a form of self maintenance plays important roles in cell survival and death. HIV-1 gp120 is reported to induce autophagy in a variety of cells. However, the effect of gp120 on autophagy in cardiomyocytes has not been reported. This study aimed to test our hypothesis that gp120 could induce autophagy in cardiomyocytes.MethodsRat cardiomyocyte H9c2 cells were treated with gp120 (100 ng/ml) in vitro for 4 h, 1 day and 7 days. The autophagy related proteins were analyzed by Western blot and the autophagosomes were analyzed by confocal microscopy.ResultsThe autophagic proteins and autophagosomes were markedly increased in the H9c2 cells after 4 h of gp120 treatment. Furthermore, gp120 induced autophagic proteins and autophagosomes were significantly inhibited by the N-methyl-d-aspartic acid (NMDA) receptor inhibitor MK801, c-Jun N-terminal kinase (JNK) inhibitor SP600125, and the class III phosphoinositide 3-kinase (PI3K) inhibitor 3-methyladenine (3-MA), while there was no change in the cells pretreated with the CXCR4 antagonist AMD3100. In addition, no apparent cell death was observed in the cardiomyocytes treated with gp120 for up to 7 days.ConclusionsIn summary, our research demonstrated for the first time that HIV-1 gp120 could induce autophagy of cardiomyocytes and the NMDA receptor, JNK and class III PI3K were involved in this process. This observation provides a new insight into the mechanisms of in the cardiovascular involvement during HIV-1 infection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Cardiology - Volume 167, Issue 6, 10 September 2013, Pages 2517-2523
نویسندگان
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