کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
59982 1419453 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzymatic resolution of epichlorohydrin catalyzed by whole cells in an organic solvent/buffer biphasic system
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Enzymatic resolution of epichlorohydrin catalyzed by whole cells in an organic solvent/buffer biphasic system
چکیده انگلیسی

The biocatalytic resolution of (R,S)-epichlorohydrin to (R)-epichlorohydrin by whole cells from a recombinant Escherichia coli BL21(ED3) expressing epoxide hydrolase activity in a series of organic solvent/buffer biphasic systems was studied. Isooctane was found to be the best organic solvent. The optimum volume ratio of the organic phase to the aqueous phase, reaction temperature, pH, substrate concentration, cell concentration, and reaction time were 7:3, 30 °C, 8.0, 574 mmol/L, 0.07 g/ml wet cells, and 45 min, respectively. Under the optimized conditions, a yield of 37.5% with 99.3% ee and an average productivity of 0.286 mol/(L·h) were obtained in a stirred reactor (1-L scale). Thus, the bioconversion was more efficient in the isooctane/buffer biphasic system than that in a monophasic aqueous system, where the substrate concentration and the average productivity of (R)-epichlorohydrin were improved by 55.2% and 98.6%, respectively.

An isooctane/buffer biphasic system is useful for dissolving the substrate epichlorohydrin (ECH) and suppressing its non-enzymatic hydrolysis. This biphasic system could prove useful for improving the concentration of substrate and yield of (R)-ECH.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Catalysis - Volume 34, Issue 7, July 2013, Pages 1339–1347
نویسندگان
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