کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
606564 | 1454535 | 2015 | 9 صفحه PDF | دانلود رایگان |
• This article shows preparation of highly charged NPs for the purpose of enhanced protein binding.
• The non-monotonic effect of ionic strength was observed and studied by qualitative and quantitative experiments.
• The protein charge anisotropy and electrostatic repulsion among NPs was concluded to control the non-monotonic ionic strength effects together.
Poly(2-(methacryloyloxy)ethyltrimethyl ammonium chloride) (PMATAC) modified magnetic nanoparticles (NPs) with a high zeta potential of ca. 50 mV were synthesized by atom transfer radical polymerization (ATRP). The prepared NPs consist of a magnetic core around 13 nm and a PMATAC shell around 20 nm attached on the surface of magnetic nanoparticles. Thermodynamic binding parameters between β-lactoglobulin and these polycationic NPs were investigated at different ionic strengths by high-resolution turbidimetry, dynamic light scattering (DLS), and isothermal titration calorimetry (ITC). Both turbidity and ITC show that binding affinities for BLG display a non-monotonic ionic strength dependence trend and a maximum appears at ionic strength of 50 mM. Such observation should arise from the coeffects of protein charge anisotropy visualized by DelPhi electrostatic modeling and the strong electrostatic repulsion among highly charged NPs at a variety of ionic strengths.
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Journal: Journal of Colloid and Interface Science - Volume 460, 15 December 2015, Pages 221–229