کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
607500 | 1454582 | 2014 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Polyvinylamine-g-galactose is a route to bioactivated silica surfaces Polyvinylamine-g-galactose is a route to bioactivated silica surfaces](/preview/png/607500.png)
• Lactobionic acid condensation with polyvinylamine gives PVAm-GAL.
• PVAm-GAL irreversibly adsorbs onto silica giving a galactose rich cationic surface.
• Phenylboronic acid derivatized PVAm complexes with PVAm-GAL.
Polyvinylamine (PVAm) was derivatized with lactobionic acid to give PVAm-GAL with pendant galactose groups along the PVAm chain. The galactose substituents were shown to undergo two types of specific interactions: (1) condensation with phenylboronic acid moieties on polymers and on surfaces; and, (2) the specific binding of RCA120, a galactose-specific lectin. Surface binding and assembly was monitored with Quartz Crystal Microbalance (QCM-D) measurements. Multilayer assemblies based on boronic ester formation were destroyed by lowering the pH, or by introducing sorbitol. We propose that the physical adsorption of PVAm-GAL onto silica or other negatively charged support surfaces is a simple route to galactose-rich interfaces, possibly useful for affinity separations, cell targeting and cell culturing.
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Journal: Journal of Colloid and Interface Science - Volume 413, 1 January 2014, Pages 86–91