A method of printing uniform protein lines by using flat PDMS stamps

In this paper, we report a method of printing uniform protein lines on glass slides by using UV-treated flat PDMS stamps. Unlike traditional microcontact printing (μCP) which requires microstructured PDMS stamps, this μCP method only requires a flat PDMS stamp, an UV lamp and a number of straight needles. Our results show that lines of bovine serum albumin (BSA), immunoglobin (IgG), anti-biotin, anti-human IgG and anti-mouse IgG can be printed evenly on glass slides by using this μCP method. We also demonstrate that the printed protein lines are suitable for applications such as microfluidic immunoassays.

We developed a method of printing uniform protein lines on glass slides by using UV-treated flat PDMS stamps. The printed protein lines were suitable for applications such as microfluidic immunoassays.Figure optionsDownload high-quality image (104 K)Download as PowerPoint slideResearch highlights
► Protein lines were evenly printed on glass slides by using UV-treated flat PDMS stamps.
► This new method is simple and inexpensive comparing to traditional microcontact printing (μCP).
► The printed protein lines were suitable for applications such as microfluidic immunoassays.