کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6160830 | 1249353 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biphasic recruitment of microchimeric fetal mesenchymal cells in fibrosis following acute kidney injury
ترجمه فارسی عنوان
استخراج دوفاز سلولهای مزانشیمی جنین میکرو شیمریک در فیبروز پس از آسیب حاد کلیه
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کلمات کلیدی
اسید آریستولچیک، میکروشیمیایی جنین، سلول های مزانشیمی، نفروپاتی فیبروز بینابینی کلیوی،
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای کلیوی
چکیده انگلیسی
Fetal microchimeric cells (FMCs) enter the maternal circulation and persist in tissue for decades. They have capacity to home to injured maternal tissue and differentiate along that tissue's lineage. This raises the question of the origin(s) of cells transferred to the mother during pregnancy. FMCs with a mesenchymal phenotype have been documented in several studies, which makes mesenchymal stem cells an attractive explanation for their broad plasticity. Here we assessed the recruitment and mesenchymal lineage contribution of FMCs in response to acute kidney fibrosis induced by aristolochic acid injection. Serial in vivo bioluminescence imaging revealed a biphasic recruitment of active collagen-producing FMCs during the repair process of injured kidney in post-partum wild-type mothers that had delivered transgenic pups expressing luciferase under the collagen type I-promoter. The presence of FMCs long-term post injury (day 60) was associated with profibrotic molecules (TGF-β/CTGF), serum urea levels, and collagen deposition. Immunostaining confirmed FMCs at short term (day 15) using post-partum wild-type mothers that had delivered green fluorescent protein-positive pups and suggested a mainly hematopoietic phenotype. We conclude that there is biphasic recruitment to, and activity of, FMCs at the injury site. Moreover, we identified five types of FMC, implicating them all in the reparative process at different stages of induced renal interstitial fibrosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Kidney International - Volume 85, Issue 3, March 2014, Pages 600-610
Journal: Kidney International - Volume 85, Issue 3, March 2014, Pages 600-610
نویسندگان
Edwige Roy, Elke Seppanen, Rebecca Ellis, Eddy S. Lee, Kiarash Khosroterani, Nicholas M. Fisk, George Bou-Gharios,