کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6173687 | 1599803 | 2014 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: An evidence in vitro for the influence of bisphenol A on uterine leiomyoma An evidence in vitro for the influence of bisphenol A on uterine leiomyoma](/preview/png/6173687.png)
ObjectiveTo study the effect and mechanism of environmental estrogens bisphenol A (BPA) on uterine leiomyoma (UL) cells.MethodsPrimary cultures and subcultures of human UL cells, which were identified by immunocytochemical staining with a monoclonal anti-α-smooth muscle actin antibody, were performed. The viability of cells in different concentration of bisphenol A of 24 h, 48 h and 72 h were analyzed by CCK-8. The expressions of mRNA of ERα, IGF-1 and VEGF in all groups were detected by real-time quantitative PCR assay, and then the expressions of proteins detected by Western blot assay.ResultsBPA promoted the growth of UL cells and the expressions of ERα, IGF-1 and VEGF, which had positive correlation with the concentration and action time of BPA treatment.ConclusionBPA promotes the growth of leiomyoma cells. The expressions of IGF-1, VEGF can be up-regulated by ERα, which may be possible mechanism of BPA promote the growth of leiomyoma cells.
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology - Volume 178, July 2014, Pages 80-83