کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6197681 1261939 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Early wound healing of laser in situ keratomileusis-like flaps after treatment with human corneal stromal stem cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی چشم پزشکی
پیش نمایش صفحه اول مقاله
Early wound healing of laser in situ keratomileusis-like flaps after treatment with human corneal stromal stem cells
چکیده انگلیسی

PurposeTo use a well-established organ culture model to investigate the effects of corneal stromal stem cells on the optical and biomechanical properties of corneal wounds after laser in situ keratomileusis (LASIK)-like flap creation.SettingSchool of Optometry and Vision Sciences, Cardiff University, Cardiff, Wales, United Kingdom.DesignExperimental study.MethodsThe LASIK-like flaps were produced in sheep corneas. The flap beds were treated with corneal stromal stem cells and were then replaced and allowed to heal for different periods of up to 3 weeks in organ culture. The optical transmission of the cornea, the force required to detach the flap, and the presence of myofibroblasts near the flap bed were measured.ResultsCorneal stromal stem cell-treated flap beds were statistically significantly more transparent after 3 weeks in culture than the untreated controls. At 3 weeks, the mean force necessary to detach the flap was more than twice the force required for the respective control samples. Concurrently, there were 44% activated cells immediately below the flap margin of the controls compared with 29% in the same region of the corneal stromal stem cell-treated flaps.ConclusionsIn this system, the presence of corneal stromal stem cells at the wound margin significantly increased the adherence of LASIK-like flaps while maintaining corneal transparency. It is postulated that this is achieved by the deposition of extracellular connective tissue similar to that found in the normal cornea and by the paucity of activated keratocytes (myofibroblasts), which are known to scatter a significant amount of the incident light.Financial DisclosureNo author has a financial or proprietary interest in any material or method mentioned.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cataract & Refractive Surgery - Volume 42, Issue 2, February 2016, Pages 302-309
نویسندگان
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