کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6219103 | 1607432 | 2016 | 8 صفحه PDF | دانلود رایگان |
ObjectivesTo assess if peripheral T cell populations in children with chronic hepatitis C virus (HCV) infection would show evidence of activation/exhaustion and an attenuated functional response.Study designCompared with adults, children with HCV infection have a higher rate of spontaneous viral clearance. In adults, chronic HCV has been linked to T cell exhaustion. Little is known of the immune status of children with HCV. Peripheral blood mononuclear cells were isolated from 16 children with HCV (6 males, 10 females; mean age 8.6 years, range 2-17), 16 age- and sex-matched control children without HCV infection, and 20 adults with chronic HCV. Multiparameter flow cytometry was performed to characterize T cell differences across the 3 groups.ResultsControls and children with HCV had similar levels of CD4+, CD8+, and γδ+ T cells. Children with HCV demonstrated a decrease in naïve T cells compared with control children and increased activation/exhaustion marker expression on both CD8+ and CD4+ T cells. Transcription factor analysis suggested functional activation of T cells in children with HCV; however, only the CD4+ subset had enhanced cytokine production (interferon gamma and interleukin-2) compared with control children.ConclusionsThe HCV response in children is characterized by several changes in T cell phenotype. Many of these changes, such as increased T cell expression of programmed cell death-1, are similar to responses in adults. Of note, cytokine production by CD4+ helper T cells is increased in children with HCV compared with age- and sex-matched control children, which may influence long-term prognosis in children with HCV.
Journal: The Journal of Pediatrics - Volume 170, March 2016, Pages 142-148.e1