کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6255854 | 1612920 | 2016 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Research report2D-gel based proteomics unravels neurogenesis and energetic metabolism dysfunction of the olfactory bulb in CUMS rat model Research report2D-gel based proteomics unravels neurogenesis and energetic metabolism dysfunction of the olfactory bulb in CUMS rat model](/preview/png/6255854.png)
- Using 2-D gel electrophoresis coupled with mass spectrometry to characterize the Olfactory Bulb under CUMS treatment.
- 29 out of 47 differential proteins were identified.
- 7 differential proteins were ruled out to validate guided by KEGG and IPA biological analysis.
- Global proteomics Characterization of OB may unravel the dysfunction under CUMS treatment.
Major depression is a devastating psychiatric disease worldwide currently. A reduced olfactory sensitivity in MDD patients was well evidenced. We previously interrogated the mechanism of decreasing hippocampus neurogenesis in CUMS rat model of depression. The Olfactory Bulb (OB) is crucial part of the olfactory system which functions in post-developmental neurogenesis. However, the mechanism of the dysfunction of OB induced by CUMS is still largely unknown. Herein, by using the chronic unpredictable mild stress (CUMS) rat model of depression, differential protein expression between the OB proteomes of CUMS and control group was interrogated through two-dimensional electrophoresis coupling with matrix-assisted laser desorption ionization-time of flight tandem mass spectrometry. Twenty nine differential protein expression was analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway over-representation and Ingenuity pathways analysis (IPA). Seven identified differential proteins were selected for Western blotting validation. This study provides insight that neurogenesis and Energy metabolism disorder is involved in OB dysfunction induced by CUMS.
Journal: Behavioural Brain Research - Volume 313, 15 October 2016, Pages 302-309