Short communicationAstrocyte morphology after ischemic and hemorrhagic experimental stroke has no influence on the different recovery patterns

- Long-term astrocyte morphology has no influence on the different recovery patterns of stroke.
- Ischemic and hemorrhagic stroke subtypes have similar long-term astrocyte morphology in perilesional sensorimotor cortex and dorsolateral striatum.
- Long-term GFAP immunoreactivity profile is similar between ischemic and hemorrhagic stroke.

Stroke, broadly subdivided into ischemic and hemorrhagic subtypes, is a serious health-care problem worldwide. Previous studies have suggested ischemic and hemorrhagic stroke could present different functional recovery patterns. However, little attention has been given to this neurobiological finding. Coincidently, astrocyte morphology could be related to improved sensorimotor recovery after skilled reaching training and modulated by physical exercise and environmental enrichment. Therefore, it is possible that astrocyte morphology might be linked to differential recovery patterns between ischemic and hemorrhagic stroke. Thus, we decided to compare long-term GFAP-positive astrocyte morphology after ischemic (IS, n = 5), hemorrhagic (HS, n = 5) and sham (S, n = 5) stroke groups (induced by endothelin-1, collagenase type IV-S and salina, respectively). Our results showed ischemic and hemorrhagic stroke subtypes induced similar long-term GFAP-positive astrocyte plasticity (P > 0.05) for all evaluated measures (regional and cellular optical density; astrocytic primary processes ramification and length; density of GFAP positive astrocytes) in perilesional sensorimotor cortex and striatum. These interesting negative results discourage similar studies focused on long-term plasticity of GFAP-positive astrocyte morphology and recovery comparison of stroke subtypes.