کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6258553 1612973 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research reportHuman bone marrow mesenchymal stem/stromal cells produce efficient localization in the brain and enhanced angiogenesis after intra-arterial delivery in rats with cerebral ischemia, but this is not translated to behavioral recovery
ترجمه فارسی عنوان
سلول های بنیادی / استرومای مزانشیمی مغز استخوان مغز انسان تولید مکانیزم کارآمد در مغز و افزایش آنژیوژنز پس از تحویل داخل شریان در موش های مبتلا به ایسکمی مغزی، اما این به بهبود رفتاری منتقل نمی شود
کلمات کلیدی
آنژیوژنز، بازیابی رفتاری، درمان سلولی، ایسکمی مغزی، سلول های مزانشیمی مشتق از مغز استخوان انسان، تحویل داخل عضلانی،
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب رفتاری
چکیده انگلیسی


- Intra-arterial cell infusion of human mesenchymal cells is efficient technique to target cells into the ischemic hemisphere.
- Intra-arterial cell delivery enhances the perilesional angiogenesis in MCAO rats.
- Cell treatment does not improve functional outcome, when strict behavioral tests were applied.

Intravascular cell therapy is a promising approach for the treatment of stroke. However, high accumulation of cells to lungs and other filtering organs is a major concern after intravenous (i.v.) cell transplantation. This can be circumvented by intra-arterial (i.a.) cell infusion, which improves homing of cells to the injured brain. We studied the effect of i.a. delivery of human bone marrow-derived mesenchymal cells (BMMSCs) on behavioral and histological outcome in rats after middle cerebral artery occlusion (MCAO). Sixty male Wistar rats were subjected to transient MCAO (60 min) or sham-operation. BMMSCs (1 × 106) were infused into the external carotid artery on postoperative day 2 or 7. Histology performed after a 42-day follow-up did not detect any human cells (MAB1281) in the ischemic brain. Endothelial cell staining with RECA-1 revealed a significant increase in the number of blood vessels in the perilesional cortex in MCAO rats treated with cells on postoperative day 7. Behavioral recovery as assessed in three tests, sticky label, cylinder and Montoya's staircase, was not improved by human BMMSCs during the follow-up. In conclusion, human BMMSCs did not improve functional recovery in MCAO rats despite effective initial homing to the ischemic hemisphere and enhanced angiogenesis, when strict behavioral tests not affected by repeated testing and compensation were utilized.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Behavioural Brain Research - Volume 259, 1 February 2014, Pages 50-59
نویسندگان
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