کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6263006 1613823 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportPositive regulation of neocortical synapse formation by the Plexin-D1 receptor
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research ReportPositive regulation of neocortical synapse formation by the Plexin-D1 receptor
چکیده انگلیسی


- New semi-automated plug-in for quantitative analysis of synapse formation in vitro.
- Plexin-D1 signaling positively mediates synapse formation in neocortical neurons.
- Adjusting levels of Plexin-D1 are predictive of increased decreased synapse formation.

Synapse formation is a critical process during neural development and is coordinated by multiple signals. Several lines of evidence implicate the Plexin-D1 receptor in synaptogenesis. Studies have shown that Plexin-D1 signaling is involved in synaptic specificity and synapse formation in spinal cord and striatum. Expression of Plexin-D1 and its principal neural ligand, Sema3E, by neocortical neurons is temporally and spatially regulated, reaching the highest level at the time of synaptogenesis in mice. In this study, we examined the function of Plexin-D1 in synapse formation by primary neocortical neurons in vitro. A novel, automated image analysis method was developed to quantitate synapse formation under baseline conditions and with manipulation of Plexin-D1 levels. shRNA and overexpression manipulations caused opposite changes, with reduction resulting in less synapse formation, an effect distinct from that reported in the striatum. The data indicate that Plexin-D1 operates in a cell context-specific fashion, mediating different synaptogenic outcomes depending upon neuron type.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1616, 7 August 2015, Pages 157-165
نویسندگان
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