کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6263241 1613853 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportSex-specific basal and hypoglycemic patterns of in vivo caudal dorsal vagal complex astrocyte glycogen metabolic enzyme protein expression
ترجمه فارسی عنوان
گزارش تحقیق ویژگی های پایه و هیپوگلیسمیک خاص ویژگی های بیان پروتئین آنزیم متابولیک گلوکوزین آستروسیت،
کلمات کلیدی
پروتئین اسیدی فیبریلاسیون گلیال، میکرو دیسکسیون لیزر مایع هیپوگلیسمی ناشی از انسولین، گلیکوژن سنتاز، گلیکوژن فسفریلاز،
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- Hindbrain astrocyte GS and GP protein expression was higher in females versus males.
- Hypoglycemia (HYPO) reduced GS and elevated GSK-3-beta proteins in each sex.
- HYPO elevated GP and decreased PP1 protein expression in males only.
- In males, lactate (L) plus HYPO augmented GS and GBE, and normalized GP.
- In females, L plus HYPO did not alter GP, but inhibited GDE.

Astrocytes contribute to neurometabolic stability through uptake, catabolism, and storage of glucose. These cells maintain the major brain glycogen reservoir, which is a critical fuel supply to neurons during glucose deficiency and increased brain activity. We used a combinatory approach incorporating immunocytochemistry, laser microdissection, and Western blotting to investigate the hypothesis of divergent expression of key enzymes regulating glycogen metabolism and glycolysis during in vivo normo- and/or hypoglycemia in male versus female hindbrain astrocytes. Glycogen synthase (GS) and glycogen phosphorylase (GP) levels were both enhanced in dorsal vagal complex astrocytes from vehicle-injected female versus male controls, with incremental increase in GS exceeding GP. Insulin-induced hypoglycemia (IIH) diminished GS and increased glycogen synthase kinase-3-beta (GSK3β) expression in both sexes, but decreased phosphoprotein phosphatase-1 (PP1) levels only in males. Astrocyte GP content was elevated by IIH in male, but not female rats. Data reveal sex-dependent sensitivity of these enzyme proteins to lactate as caudal hindbrain repletion of this energy substrate fully or incompletely reversed hypoglycemic inhibition of GS and prevented hypoglycemic augmentation of GSK3β and GP in females and males, respectively. Sex dimorphic patterns of glycogen branching and debranching enzyme protein expression were also observed. Levels of the rate-limiting glycolytic enzyme, phosphofructokinase, were unaffected by IIH with or without lactate repletion. Current data demonstrating sex-dependent basal and hypoglycemic patterns of hindbrain astrocyte glycogen metabolic enzyme expression imply that glycogen volume and turnover during glucose sufficiency and shortage may vary accordingly.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1586, 24 October 2014, Pages 90-98
نویسندگان
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