کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6263582 1613902 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportHemodynamic changes during neural deactivation in awake mice: A measurement by laser-Doppler flowmetry in crossed cerebellar diaschisis
ترجمه فارسی عنوان
تحقیقات تحقیقاتی تغییرات هومودینامیک در زمان غیرفعال کردن عصب در موش های بیداری: اندازه گیری با استفاده از روش لیزر-داپلر در دیسکسیس مخچه مخفی
کلمات کلیدی
جریان خون شهری، سرعت سلول گلبول قرمز، غلظت گلبول قرمز، غیر فعال کردن نورون بیدار شدن حیوانات، دیسکسیس مخچه مخروطی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- Developed an awake mouse model of neural deactivation in the cerebellum after MCAO.
- Reduction of CbBF was observed one day after MCAO.
- The change in RBC concentration was far greater than that in RBC velocity.
- Longitudinal experiments showed that the hemodynamic changes were reproducible.

Crossed cerebellar diaschisis (CCD) caused by contralateral supratentorial lesions can be considered a condition of neural deactivation, and hemodynamic changes in CCD were investigated with positron emission tomography (PET) in humans. In the present study, to investigate the effects of neural deactivation on hemodynamics, we developed a new mouse model of CCD, which was caused by middle cerebral artery occlusion (MCAO), and measured changes in cerebellar blood flow (CbBF), red blood cell (RBC) velocity and concentration due to CCD using laser-Doppler flowmetry (LDF) in awake mice. The ratio of the CCD side to the unaffected side in the cerebellum for CbBF 1 day after MCAO was decreased by −18% compared to baseline (before CCD). The ratio of the CCD side to the unaffected side for RBC concentration 1 day after MCAO was decreased by −23% compared to baseline. However, no significant changes in the ratio of the CCD side to the unaffected side were observed for RBC velocity. The present results indicate that the reduction of CbBF induced by neural deactivation was mainly caused by the decrease in RBC concentration. In contrast, our previous study showed that RBC velocity had a dominant role in the increase in cerebral blood flow (CBF) induced by neural activation. If RBC concentration can be considered an indicator of cerebral blood volume (CBV), hemodynamic changes due to neural activation and deactivation measured by LDF in mice might be in good agreement with human PET studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1537, 6 November 2013, Pages 350-355
نویسندگان
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