کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6263710 1613908 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportActivin A regulation under global hypoxia in developing mouse brain
ترجمه فارسی عنوان
گزارش تحقیقاتی آکتینین مقررات تحت هیپوکسی جهانی در حال توسعه مغز موش
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- Activin A, its receptors and antagonists are expressed early in postnatal developing mouse brain.
- In contrast to other insults, hypoxia alone does not up-regulate activin A in mouse brain.
- Stabilization of Hypoxia Inducible Factors (HIF) does not lead to a stimulation of the activin system.
- In complex insults like perinatal brain damage other factors than hypoxia seem to give rise to activin A overexpression.

Activin A is a multifunctional growth and differentiation factor with pronounced neuroprotective properties that is strongly up-regulated in various forms of acute brain disorders and injuries including epilepsy, stroke and trauma. In a pediatric context, activin A has been advanced as a potential marker for the severity of perinatal hypoxic-ischemic brain injury. Here we investigated the regulation of activin A under global hypoxia without ischemia in primary cultures of cortical neurons and in neonatal and adult mice of two strains (C57BL/6 and CD-1). From birth to adulthood, activin βA subunit, activin receptors, and functional activin antagonists were all expressed at roughly similar mRNA levels in the brain of C57BL/6 mice. Independent of mouse line and age, we found both moderate (11% O2, 2 h) and severe hypoxia (8%, 6 h) to be consistently associated with normal or even reduced levels of activin βA (Inhba) mRNA. The surprising unresponsiveness of Inhba expression to hypoxia was confirmed at the protein level. In situ hybridization did not indicate regional, hypoxia-related differences in Inhba expression. Pharmacologic stabilization of hypoxia inducible factors with the prolyl hydroxylase inhibitor FG-4497 did not influence Inhba mRNA levels in neonatal mice. Our data indicate that pure hypoxia differs from other, more complex types of brain damage in that it appears not to recruit activin A as an endogenous neuroprotective agent.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1531, 19 September 2013, Pages 65-74
نویسندگان
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