کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6263802 1613917 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Altered hippocampal myelinated fiber integrity in a lithium-pilocarpine model of temporal lobe epilepsy: A histopathological and stereological investigation
ترجمه فارسی عنوان
یکپارچگی فیبرهای میلینین هیپوکامپ تغییر یافته در یک مدل لیتیوم-پیلوکارپین صرع لوب تمپورال: بررسی هیستوپاتولوژی و استریولوژیک
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


• Myelin sheath damage occurred in the hippocampus of epilepsy rats.
• Total volume of hippocampal myelinated fibers was reduced in epilepsy rats.
• Total volume of myelin sheaths in the hippocampus was decreased in epilepsy rats.
• Thickness of myelin sheaths was less, especially for small fibers, in epilepsy rats.
• Total length of myelinated fibers was less in the hippocampus of epilepsy rats.

The damage of white matter, primarily myelinated fibers, in the central nervous system (CNS) of temporal lobe epilepsy (TLE) patients has been recently reported. However, limited data exist addressing the types of changes that occur to myelinated fibers inside the hippocampus as a result of TLE. The current study was designed to examine this issue in a lithium-pilocarpine rat model. Investigated by electroencephalography (EEG), Gallyas silver staining, immunohistochemistry, western blotting, transmission electron microscopy, and stereological methods, the results showed that hippocampal myelinated fibers of the epilepsy group were degenerated with significantly less myelin basic protein (MBP) expression relative to those of control group rats. Stereological analysis revealed that the total volumes of hippocampal formation, myelinated fibers, and myelin sheaths in the hippocampus of epilepsy group rats were decreased by 20.43%, 49.16%, and 52.60%, respectively. In addition, epilepsy group rats showed significantly greater mean diameters of myelinated fibers and axons, whereas the mean thickness of myelin sheaths was less, especially for small axons with diameters from 0.1 to 0.8 µm, compared to control group rats. Finally, the total length of the myelinated fibers in the hippocampus of epilepsy group rats was significantly decreased by 56.92%, compared to that of the control group, with the decreased length most prominent for myelinated fibers with diameters from 0.4 to 0.8 µm. This study is the first to provide experimental evidence that the integrity of hippocampal myelinated fibers is negatively affected by inducing epileptic seizures with pilocarpine, which may contribute to the abnormal propagation of epileptic discharge.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1522, 19 July 2013, Pages 76–87