کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6263978 | 1613947 | 2013 | 6 صفحه PDF | دانلود رایگان |

We used isolated chicken retina to induce spreading depression by the glutamate receptor agonist N-methyl-d-aspartate. The N-methyl-d-aspartate-induced latency time of spreading depression was extended by the glycineB binding site competitive antagonist 7-chlorokynurenic acid. Addition of the glycine transporter type-1 inhibitors NFPS and Org-24461 reversed the inhibitory effect of 7-chlorokynurenic acid on N-methyl-d-aspartate-evoked spreading depression. The glycine uptake inhibitory activity of Org-24461, NFPS, and some newly synthesized analogs of NFPS was determined in CHO cells stably expressing human glycine transporter type-1b isoform. Compounds, which failed to inhibit glycine transporter type-1, also did not have effect on retinal spreading depression. These experiments indicate that the spreading depression model in chicken retina is a useful in vitro test to determine activity of glycine transporter type-1 inhibitors. In addition, our data serve further evidence for the role of glycine transporter type-1 in retinal neurotransmission and light processing.
⺠The glutamate receptor agonist NMDA induced spreading depression in chick retina. ⺠7-Chlorokynurenic acid extended the latency NMDA-induced spreading depression. ⺠The GlyT-1 inhibitors NFPS and Org-24461 reversed the effect of 7-chlorokynurenic acid. ⺠GlyT1 regulating glycine levels in the retina may have a role in light processing.
Journal: Brain Research - Volume 1492, 25 January 2013, Pages 1-6