کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6264118 | 1613959 | 2012 | 8 صفحه PDF | دانلود رایگان |
The central nervous system (CNS) in adult mammals loses the ability to regenerate after injury. Although electrical stimulation (ES) has been shown to promote neural regeneration, the underlying mechanisms by which ES enhances CNS regeneration remain elusive. The aim of the present study was to investigate the effect of ES on neurite outgrowth of goldfish retinal explants. The optic nerve of adult goldfish was intraorbitally crushed 7-14 days before retinal explants preparation. The explants were cultured in a laminin-coated indium tin oxide (ITO, transparent conducting oxide) device for ES application. Various strengths and waveforms of ES were applied to examine their effect on neural outgrowth. When the retinal explants were stimulated for 1Â h with intermittent pulses on day 1 (20Â Hz; 10 pulses every 2Â s), the regenerated neurite length was significantly increased compared to explants that were stimulated with continuous square waves or continuous pulses over the same time course. It was also found that increased ES strength and repeated ES each day can enhance neurite outgrowth significantly. These results suggest that intermittent pulse ES is able to promote neurite regeneration most effectively in goldfish retinal explants, and that this electrically stimulated neurite outgrowth using ITO conductive electrodes may provide a useful platform for investigating cellular mechanisms of CNS regeneration.
⺠Electrical stimulation (ES) on neurite outgrowth of goldfish retinal explants was studied. ⺠Intermittent pulse ES is able to promote neurite regeneration most effectively. ⺠This ES promoted neurite outgrowth using ITO provides a platform for studying CNS regeneration.
Journal: Brain Research - Volume 1480, 22 October 2012, Pages 22-29