کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6264201 1613967 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportIntrathecal PLCβ3 oligodeoxynucleotides antisense potentiates acute morphine efficacy and attenuates chronic morphine tolerance
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research ReportIntrathecal PLCβ3 oligodeoxynucleotides antisense potentiates acute morphine efficacy and attenuates chronic morphine tolerance
چکیده انگلیسی

Morphine is a mainstay for chronic pain treatment, but its efficacy has been hampered by physical tolerance. The underlying mechanism for chronic morphine induced tolerance is complicated and not well understood. PLCβ3 is regarded as an important factor in the morphine tolerance signal pathway. In this study, we determined intrathecal (i.t.) administration of an antisense oligodeoxynucleotide (ODN) of PLCβ3 could quicken the on-set antinociceptive efficacy of acute morphine treatment and prolong the maximum effect up to 4 h. The antisense could also attenuate the development of morphine-induced tolerance and left shift the ED50 after 7 day of coadministration with morphine. These results probably were contributed by the PLCβ3 antisense ODN as they successfully knocked down protein expression levels and reduced activity of PLCβ3 in spinal cord in rats. The mismatch group had no such effects. The results confirmed the important involvement of PLCβ3 in both acute morphine efficacy and chronic morphine tolerance at spinal level in rats. This study may provide an idea for producing a novel adjuvant for morphine treatment.

► PLCβ3 antisense reduced the PLCβ3 protein level and activity in spinal cord in rats. ► PLCβ3 antisense potentiated acute morphine antinociceptive efficacy in rats. ► PLCβ3 antisense attenuated chronic i.t. morphine tolerence in rats.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1472, 7 September 2012, Pages 38-44
نویسندگان
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