Research ReportA substance P antagonist improves outcome when administered 4Â h after onset of ischaemic stroke

Previous studies have suggested that substance P (SP) plays a critical role in the development of brain oedema and functional deficits following traumatic brain injury and that SP receptor antagonism may improve outcome. No studies have described such a role in ischemic stroke. The present study characterized the effects of the NK1 tachykinin receptor antagonist, n-acetyl-l-tryptophan (NAT), on blood-brain barrier (BBB) breakdown, oedema formation, infarct volume and functional outcome following reversible ischemic stroke in rats. Ischemia was induced using a reversible thread model of middle cerebral artery occlusion where occlusion was maintained for 2 h before reperfusion. Animals received either NAT or equal volume saline vehicle intravenously at 2 h post-reperfusion. Ischaemic stroke resulted in increased perivascular SP immunoreactivity at 24 h. Administration of NAT significantly reduced oedema formation and BBB permeability at 24 h post-ischemia and significantly improved functional outcome as assessed over 7 days. There was no effect on infarct volume. We conclude that inhibition of SP activity with a NK1 tachykinin receptor antagonist is effective in reducing cerebral oedema, BBB permeability and functional deficits following reversible ischemia and may therefore represent a novel therapeutic approach to the treatment of ischaemic stroke.

Research highlights► Ischemic stroke significantly increases ipsilateral substance P expression. ► An NK1 tachykinin receptor antagonist prevented blood-brain barrier dysfunction. ► An NK1 tachykinin receptor antagonist prevented the genesis of cerebral oedema. ► An NK1 tachykinin receptor antagonist reduced functional deficits. ► Inhibition of neurogenic inflammation may be a novel target for ischemic stroke.