کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6267945 1614609 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The in vitro isolated whole guinea pig brain as a model to study epileptiform activity patterns
ترجمه فارسی عنوان
سرماخوردگی کامل خوکچه دریایی را به عنوان یک مدل برای مطالعه الگوهای فعالیت در معرض انقباض در نمونه آزمایشگاهی آزمایش می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- The in vitro isolated brain retains the connectivity between remote brain areas.
- Acute epileptiform activities can be induced by simple pharmacological manipulations.
- Epileptiform patterns determinants can be identified in a close-to-in vivo condition.

BackgroundResearch on ictogenesis is based on the study of activity between seizures and during seizures in animal models of epilepsy (chronic condition) or in in vitro slices obtained from naïve non-epileptic brains after treatment with pro-convulsive drugs, manipulations of the extracellular medium and specific stimulation protocols.New methodThe in vitro isolated guinea pig brain retains the functional connectivity between brain structures and maintains interactions between neuronal, glial and vascular compartments. It is a close-to-in vivo preparation that offers experimental advantages not achieved with the use of other experimental models. Neurophysiological and imaging techniques can be utilized in this preparation to study brain activity during and between seizures induced by pharmacological or functional manipulations.ResultsCellular and network determinants of interictal and ictal discharges that reproduce abnormal patterns observed in human focal epilepsies and the associated changes in extracellular ion and blood-brain permeability can be identified and analyzed in the isolated guinea pig brain.Comparison with existing methodsIctal and interictal patterns recorded in in vitro slices may show substantial differences from seizure activity recorded in vivo due to slicing procedure itself. The isolated guinea pig brain maintained in vitro by arterial perfusion combines the typical facilitated access of in vitro preparations, that are difficult to approach during in vivo experiments, with the preservation of larger neuronal networks.ConclusionsThe in vitro whole isolated guinea pig brain preparation offers an unique experimental model to study systemic and neurovascular changes during ictogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 260, 15 February 2016, Pages 83-90
نویسندگان
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