کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6268094 1614612 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Basic neuroscienceVersatile 3D-printed headstage implant for group housing of rodents
ترجمه فارسی عنوان
ایمپلنت عصب پایه ایمپلنت هدایت سه بعدی چاپی برای مسکن گروهی از جوندگان
کلمات کلیدی
جداسازی، تندرستی، جوندگان ایمپلنت جمجمه، محیط اجتماعی، مسکن جفتن
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- A novel 3D-printed headstage was developed for protecting skull-mounted implants in rodents.
- The socket allowed for successful chronic pair-housing of rats following stereotaxic surgery.
- Rats were able to carry out a range of normal behaviours, with no significant implant damage observed.
- This implant can help to improve the well-being of post-surgical rats, whilst reducing the cost of rodent upkeep.

BackgroundAn unfavourable yet necessary side-effect of stereotaxic surgery involves the social isolation of post-surgery rats, in order to protect their wound site or skull-mounted implant from damage. Social isolation can cause a myriad of behavioural and physiological changes that are detrimental to the well-being of rats, with potential negative implications for a range of experimental paradigms.New method. Female Sprague Dawley rats (n = 40) were implanted onto the skull with a novel 3D-printed headstage socket that surrounded an electrode connector. The socket accommodated a removable stainless-steel headcap for the purposes of protecting the implant. Rats were pair-housed following surgery, and their behaviour was monitored for up to several weeks under two experimental conditions that involved EEG recording and deep-brain stimulation, as well as behavioural test sessions inside an open-field maze. Rat weights were compared between individually- and pair-housed rats at up to 3 weeks post-surgery.ResultsThese experiments were successfully carried out using pair-housed rats, with no damage or complications observed regarding the implant and its headcap. Rats were able to carry out a range of normal behaviours including running, grooming, foraging and sleeping. Compared to individually-housed rats, pair-housed rats gained less weight over the 3 weeks post-implantation period.Comparison with existing method(s). This method offers additional protection compared to group-housed post-surgical rats that lack the protective headcap. It is also potentially more practical and versatile than a fully-implantable device for the safe post-surgery group housing of rodents.ConclusionsThis implant design can reduce the cost of rodent upkeep, whilst potentially avoiding a myriad of behavioural and physiological changes that are known to result from social isolation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 257, 15 January 2016, Pages 134-138
نویسندگان
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