Basic NeuroscienceValidation of video motion-detection scoring of forced swim test in mice

- We use a video motion-detection method in MATLAB to quantify the forced swim test.
- Data obtained by computer scoring correlate well with visual scoring.
- Group differences detected by computer and visual scoring are almost the same.
- Hybrid F1 mice are more active than parental strains in the test.

BackgroundThe forced swim test (FST) is used to predict the effectiveness of novel antidepressant treatments. In this test, a mouse or rat is placed in a beaker of water for several minutes, and the amount of time spent passively floating is measured; antidepressants reduce the amount of such immobility. Though the FST is commonly used, manually scoring the test is time-consuming and involves considerable subjectivity.New methodWe developed a simple MATLAB-based motion-detection method to quantify mice's activity in videos of FST. FST trials are video-recorded from a side view. Each pixel of the video is compared between subsequent video frames; if the pixel's color difference surpasses a threshold, a motion count is recorded.ResultsHuman-scored immobility time correlates well with total motion detected by the computer (r = −0.80) and immobility time determined by the computer (r = 0.83). Our computer method successfully detects group differences in activity between genotypes and different days of testing. Furthermore, we observe heterosis for this behavior, in which (C57BL/6J × A/J) F1 hybrid mice are more active in the FST than the parental strains.Comparison with existing methodsThis computer-scoring method is much faster and more objective than human scoring. Other automatic scoring methods exist, but they require the purchase of expensive hardware and/or software.ConclusionThis computer-scoring method is an effective, fast, and low-cost method of quantifying the FST. It is validated by replicating statistical differences observed in traditional visual scoring. We also demonstrate a case of heterosis in the FST.