Validation of the isotropic fractionator: Comparison with unbiased stereology and DNA extraction for quantification of glial cells

Since the IF revealed about 100% of the numbers produced by unbiased stereology, there is no significant underestimate of glial cells. This confirms the notion that the human brain overall contains glial cells and neurons with a ratio of about 1:1 - far from the originally assumed ratio of 10:1 in favor of glial cells.