Single electrode dynamic clamp with StdpC

► A novel implementation of active electrode compensation is introduced for the soft-real time dynamic clamp software StdpC to allow artefact-free dynamic clamp with a single, potentially high impedance, electrode. ► The method and its implementation are tested on an electronic model cell and in two different electrophysiological preparations. ► For the first time, active electrode compensation has been successfully verified with a second, independent electrode both in an electronic substitute cell and in a biological neuron. ► The new method is provided as part of the free software StdpC and comes with semi-automated calibration methods that allow its application by non-expert users.