کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6269094 1295119 2013 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
FACS binding assay for analysing GDNF interactions
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
FACS binding assay for analysing GDNF interactions
چکیده انگلیسی
Glial cell-line derived neurotrophic factor (GDNF) is a secreted protein with great therapeutic potential. However, in order to analyse the interactions between GDNF and its receptors, researchers have been mostly dependent of radioactive binding assays. We developed a FACS-based binding assay for GDNF as an alternative to current methods. We demonstrated that the FACS-based assay using TGW cells allowed readily detection of GDNF binding and displacement to endogenous receptors. The dissociation constant and half maximal inhibitory concentration obtained were comparable to other studies using standard binding assays. Overall, this FACS-based, simple to perform and adaptable to high throughput setup, provides a safer and reliable alternative to radioactive methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 218, Issue 1, 15 August 2013, Pages 25-28
نویسندگان
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