Characterization of [3H]CFT binding to the norepinephrine transporter suggests that binding of CFT and nisoxetine is not mutually exclusive

► We examined that [3H]CFT is a valid radioligand for NET. ► We concluded binding of CFT and nisoxetine to NET is not mutually exclusive. ► Bindings of CFT and nisoxetine to HEK-NET are differently regulated by sodium concentration.