Basic Neuroscience“Beetrack”: A software for 2D open field locomotion analysis in honey bees

“Beetrack” is a complete software designed to analyze the 2D locomotor behavior of four simultaneous honey bees moving in petri dishes. Several dependent variables can be measured: traveled distance, number of jumps and % of immobility. A spatial analysis can also be performed to detect if the bees are using preferentially one part of the box. The software has been successfully tested with eight honey bees and promise to be an interesting tool in the pharmacology field.

► We propose software for 2D locomotor behavior analysis in honey bees. ► Four honey bees can be analyzed at the same time. ► The software measures several dependent variables related to traveled distances, flight attempts, immobility and spatial behavior.