A reliable in vitro model for studying peripheral nerve myelination in mouse

► The study presents a reliable protocol for BL/6 mouse dorsal-root cultures to study myelination. ► The study evaluates analytic myelin read-outs, also for studying myelination in vital cultures. ► We show an optimised rat DRG protocols with improved myelin ratio and its comparison, respectively. ► The study presents an effective method to study demyelination in the mouse model.