Development of BMP7-producing human cells, using a third generation lentiviral gene delivery system

► A third generation BMP7 lentiviral vector was developed and tested in several in vitro models. ► SMAD phosphorylation and neuronal differentiation assays were used to verify the bioactivity and functionality of the lentiviral-based BMP7. ► In vivo studies were performed to demonstrate the effectiveness of BMP7 infection in the mouse brain.