Synaptoneurosome micromethod for fractionation of mouse and human brain, and primary neuronal cultures

► Easy step-by-step protocol for preparing synaptoneurosomes (SNs) from small amounts of mammalian brain tissue. ► Novel SN applications including resected human neocortex and rat primary cortical neuronal cultures. ► New tool for synaptic biology including SNs prepared from human neurodegenerative diseases for example AD and PD.