Spike detection in human muscle sympathetic nerve activity using a matched wavelet approach

Sympathetic nerve recordings associated with blood pressure regulation can be recorded directly using microneurography. A general characteristic of this signal is spontaneous burst activity of spikes (action potentials) separated by silent periods against a background of considerable Gaussian noise. During measurement with electrodes, the raw muscle sympathetic nerve activity (MSNA) signal is amplified, band-pass filtered, rectified and integrated. This integration process removes information regarding action potential content and their discharge properties. This paper proposes a new method for detecting action potentials from the raw MSNA signal to enable investigation of post-ganglionic neural discharge properties.The new method is based on the design of a mother wavelet that is matched to an actual mean action potential template extracted from a real raw MSNA signal. To detect action potentials, the new matched wavelet is applied to the MSNA signal using a continuous wavelet transform following a thresholding procedure and finding of a local maxima that indicates the location of action potentials. The performance of the proposed method versus two previous wavelet-based approaches was evaluated using (1) real MSNA recorded from seven healthy participants and, (2) simulated MSNA. The results show that the new matched wavelet performs better than the previous wavelet-based methods that use a non-matched wavelet in detecting action potentials in the MSNA signal.

Research Highlights▶ A new matched wavelet action potential detector for analyzing human muscle sympathetic nerve activity signal. ▶ A considerable reduction in false positive alarms and improvement in correct detection of action potentials using the new matched wavelet method. ▶ A new method for simulating muscle sympathetic nerve activity. ▶ Action potential summation in multi-fiber sympathetic recordings.