کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6269965 1295169 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Early phenotypical diagnoses in Trembler-J mice model
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Early phenotypical diagnoses in Trembler-J mice model
چکیده انگلیسی

Pmp-22 mutant mice (Trembler-J: B6.D2-Pmp22/J), are used as a model to study Charcot-Marie-Tooth type 1A (CMT1A). The identification of individual genotypes is a routine in the management of the TrJ colony. The earliest phenotypic manifestation of the pmp-22 mutation is just about 20th postnatal days, when pups begin to tremble. In this study, a rapid and simple diagnostic method was developed by modifying the Tail Suspension Test (MTST) to determine the difference between the TrJ and the wild-type mice phenotype. The animal behavioral phenotypes generated during the test were consistent with the specific genotype of each animal. The MTST allowed us to infer the heterozygous genotype in early postnatal stages, at 11 days after birth.The motor impairment of TrJ mice was also analyzed by a Fixed Bar Test (FBT), which revealed the disease evolution according to age.The main advantages of MTST are its objectivity, simplicity, and from the viewpoint of animal welfare, it is a non-invasive technique that combined with his rapidity show its very well applicability for use from an early age in these mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 190, Issue 1, 30 June 2010, Pages 14-19
نویسندگان
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