کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6270056 | 1295176 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Standardized crush injury of the mouse median nerve
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The employment of transgenic mouse models for peripheral nerve regeneration studies is continuously increasing. In this paper, we describe a standardized method for inducing a crush lesion in mouse median nerve using a non-serrated clamp exerting a crush compression force of 20.43Â MPa for a duration of 30Â s. Quantitative assessment of posttraumatic functional recovery by grasping test showed that recovery was very fast and mice returned to baseline performance already after 20 days only. Stereological analysis of nerve fibers distal to the crush lesion showed the presence of axons with a significantly smaller size and thinner myelin sheath in comparison to controls. This experimental nerve injury model is highly reproducible and the impact on animal well-being is minimal. Its employment can be particularly indicated for exploring the basic neurobiological mechanisms of peripheral nerve regeneration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 188, Issue 1, 30 April 2010, Pages 71-75
Journal: Journal of Neuroscience Methods - Volume 188, Issue 1, 30 April 2010, Pages 71-75
نویسندگان
G. Ronchi, S. Raimondo, A.S.P. Varejão, P. Tos, I. Perroteau, S. Geuna,