کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6270291 | 1295204 | 2009 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Achieving synaptically relevant pulses of neurotransmitter using PDMS microfluidics Achieving synaptically relevant pulses of neurotransmitter using PDMS microfluidics](/preview/png/6270291.png)
Fast synaptic transmission is mediated by post-synaptic ligand-gated ion channels (LGICs) transiently activated by neurotransmitter released from pre-synaptic vesicles. Although disruption of synaptic transmission has been implicated in numerous neurological and psychiatric disorders, effective and practical methods for studying LGICs in vitro under synaptically relevant conditions are unavailable. Here, we describe a novel microfluidic approach to solution switching that allows for precise temporal control over the neurotransmitter transient while substantially increasing experimental throughput, flexibility, reproducibility, and cost-effectiveness. When this system was used to apply ultra-brief (â¼400 μs) GABA pulses to recombinant GABAA receptors, members of the cys-loop family of LGICs, the resulting currents resembled hippocampal inhibitory post-synaptic currents (IPSCs) and differed from currents evoked by longer, conventional pulses, illustrating the importance of evaluating LGICs on a synaptic timescale. This methodology should therefore allow the effects of disease-causing mutations and allosteric modulators to be evaluated in vitro under physiologically relevant conditions.
Journal: Journal of Neuroscience Methods - Volume 177, Issue 2, 15 March 2009, Pages 294-302