Reversible and irreversible knockout of the ventroposterolateral thalamic nucleus measured by intracerebral SEP recordings in the rat brain-An aid to neuronavigation in small nuclei

Centrally active drugs are often hard to administer because of the blood brain barrier, and frequently high systemic doses are required to reach sufficient brain parenchyma concentrations, since these drugs are, additionally, diluted in the total blood volume. Moreover, topical administration via the systemic route is not possible. We here propose a technique for the local, quantitative deposition of active substances at defined intracerebral targets, e.g. the thalamic nuclei. We used a long micropipette and stereotactically advanced it to the desired coordinates under electrophysiological control. The pipette acted as both an electrode for intracerebral recordings and as a transportation means for the drug. The amplitude of intracerebral evoked potentials relayed by the thalamic nucleus to the sensorimotor cortex indicated the distance between the pipette tip and the neurons of the targeted nucleus. Data were obtained from anesthetized rats, where the micropipette was advanced towards the nucleus ventralis posterolateralis (VPL) during contralateral electrical forepaw stimulation and intracerebral recording of somatosensory evoked potentials. Within the VPL we either injected lidocaine or kainic acid, both resulting in an attenuation of the intracerebral as well as the cortical evoked potentials. This proposed tool may be useful for functional investigations of deep brain structures.