کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6270462 1614656 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A stereo-compound hybrid microscope for combined intracellular and optical recording of invertebrate neural network activity
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
A stereo-compound hybrid microscope for combined intracellular and optical recording of invertebrate neural network activity
چکیده انگلیسی

Optical recording studies of invertebrate neural networks with voltage-sensitive dyes seldom employ conventional intracellular electrodes. This may in part be due to the traditional reliance on compound microscopes for such work. While such microscopes have high light-gathering power, they do not provide depth of field, making working with sharp electrodes difficult. Here we describe a hybrid microscope design, with switchable compound and stereo objectives, that eases the use of conventional intracellular electrodes in optical recording experiments. We use it, in combination with a voltage-sensitive dye and photodiode array, to identify neurons participating in the swim motor program of the marine mollusk Tritonia. This microscope design should be applicable to optical recording studies in many preparations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 162, Issues 1–2, 15 May 2007, Pages 148-154
نویسندگان
, , ,