کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6270527 1614657 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain
چکیده انگلیسی

The amino acid neurotransmitters γ-aminobutyric acid (GABA) and glutamate have been implicated in mood disorders and high performance liquid chromatography (HPLC) is an important method in their quantitation. This paper describes a method which employs either electrochemical or fluorescent detection of the amino acid derivatives formed by a reaction with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide ions. Elution is based on an isocratic protocol and is achieved in 20 min or less. The method is reproducible, shows excellent linearity and generates derivatives which are stable for up to 16 h. Successful application of the method to a maternal deprivation study has identified the hippocampus and the brainstem as possible sites of long-term alteration in this paradigm.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 160, Issue 2, 15 March 2007, Pages 223-230
نویسندگان
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