Cholinergic interneurons suppress action potential initiation of medium spiny neurons in rat nucleus accumbens shell

► We examined cholinergic effects on medium spiny neurons in nucleus accumbens shell. ► Carbachol depolarized resting membrane potential and increased input resistance. ► Carbachol suppressed slowly depolarizing potential and increased rheobase. ► Cholinergic neuronal stimulation also suppressed slowly depolarizing potential.