Parallel dopamine D1 receptor activity dependence of l-Dopa-induced normal movement and dyskinesia in mice

l-3,4-Dihydroxyphenylalanine (l-Dopa)-induced dyskinesia (LID) in Parkinson's disease (PD) is a major clinical problem. The prevailing view is that in PD patients and animal PD models dyskinesia develops after repeated l-dopa use or priming, independent of l-dopa's anti-PD therapeutic effect that occurs immediately. Here we show that in mice with severe and consistent dopamine (DA) loss in the dorsal striatum, rendered by transcription factor Pitx3 null mutation, the very first injection of l-dopa or D1-like agonist SKF81297 induced both normal ambulatory and dyskinetic movements. Furthermore, the robust stimulating effects on normal and dyskinetic movements had an identical time course and parallel dose-response curves. In contrast, D2-like agonist ropinirole stimulated normal and dyskinetic movements relatively modestly. These results demonstrate that severe DA loss in the dorsal striatum sets the stage for dyskinesia to occur on the first exposure to l-dopa or a D1 agonist without any priming. These results also indicate that l-dopa stimulated both normal and dyskinetic movements primarily via D1 receptor activation and that proper D1 agonism is potentially an efficacious therapy for PD motor deficits.

► DA D1 receptors are the primary mediator of l-dopa's anti-PD effect. ► Severe DA loss in the dorsal striatum sets the stage for l-dopa-induced dyskinesia. ► l-Dopa can induce dyskinesia without any priming. ► D1 receptors are the shared mechanism for l-dopa's anti-PD and dyskinetic effects. ► l-Dopa's anti-PD and dyskinetic effects are intrinsically linked.