کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6276201 1614884 2011 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regeneration, Repair, and Developmental NeuroscienceResearch PaperFunctional development of the vagal and glossopharyngeal nerve-related nuclei in the embryonic rat brainstem: optical mapping with a voltage-sensitive dye
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Regeneration, Repair, and Developmental NeuroscienceResearch PaperFunctional development of the vagal and glossopharyngeal nerve-related nuclei in the embryonic rat brainstem: optical mapping with a voltage-sensitive dye
چکیده انگلیسی

We investigated functional organization of the vagus nerve (N. X)- and glossopharyngeal nerve (N. IX)-related nuclei in the embryonic rat brainstem and compared their development and spatial distribution patterns, using multiple-site optical recording with a fast voltage-sensitive dye, NK2761. Intact brainstem preparations with N. X and N. IX attached were dissected from E13-E16 rat embryos, and electrical responses evoked by N. X/N. IX stimulation were optically recorded from many loci of the stained preparations. We analyzed optical waveforms and separated fast and slow optical signals corresponding to the antidromic/orthodromic action potentials and the excitatory postsynaptic potentials (EPSPs), respectively. We constructed contour line maps of signal amplitudes and identified motor and sensory nuclei of N. X and N. IX. In the N. X-related motor nucleus (the dorsal motor nucleus of the vagus nerve: DMNV), the fast signals were distributed in multiple-peak patterns, suggesting that the neurons and/or their activity are not distributed uniformly within the motor nuclei at early developmental stages. In the sensory nucleus (the nucleus of the tractus solitarius: NTS), the EPSPs were first detected from E15 in normal physiological solution for both N. X and N. IX. The N. IX-related NTS partially overlapped with the N. X-related NTS, but the peak locations were different between these two nerves. The results obtained in this study suggest that functional organization of the N. X- and N. IX-related nuclei changes dynamically with development in the embryonic rat brainstem.

▶We applied multiple-site optical recording with a fast voltage-sensitive dye to the embryonic rat brainstem and investigated functional organization of the vagus nerve (N. X)- and glossopharyngeal nerve (N. IX)-related nuclei. ▶We succeeded in recording optical responses evoked by N. X/N. IX stimulation and in separating fast and slow optical signals corresponding to the antidromic/orthodromic action potentials and the excitatory postsynaptic potentials (EPSPs), respectively. ▶We constructed contour line maps of time-integrated areas of optical responses and identified motor and sensory nuclei of N. X and N. IX. ▶In the N. X-related motor nucleus (the dorsal motor nucleus of the vagus nerve: DMNV), the fast signals were distributed in multiple-peak patterns, and we suggested that the neurons and/or their activity are not distributed uniformly within the motor nuclei at early developmental stages. ▶In the sensory nucleus (the nucleus of the tractus solitarius: NTS), we determined that the EPSPs were first detected from E15 in normal physiological solution for both N. X and N. IX. ▶We showed that functional organization of the N. X- and N. IX-related nuclei changes dynamically with development in the embryonic rat brainstem.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 192, 29 September 2011, Pages 781-792
نویسندگان
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