کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6276527 1614899 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Neurodegeneration, Neuroprotection, and Disease-Oriented NeuroscienceResearch PaperQuantification of reserve pool dopamine in methionine sulfoxide reductase A null mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Neurodegeneration, Neuroprotection, and Disease-Oriented NeuroscienceResearch PaperQuantification of reserve pool dopamine in methionine sulfoxide reductase A null mice
چکیده انگلیسی

Methionine sulfoxide reductase A knockout (MsrA−/−) mice, which serve as a potential model for neurodegeneration, suffer from increased oxidative stress and have previously been found to have chronically elevated brain dopamine (DA) content levels relative to control mice. Additionally, these high levels parallel the increased presynaptic DA release. In this study, fast-scan cyclic voltammetry (FSCV) at carbon-fiber microelectrodes was used to quantify striatal reserve pool DA in knockout mice and wild-type control mice. Reserve pool DA efflux, induced by amphetamine (AMPH), was measured in brain slices from knockout and wild type (WT) mice in the presence of α-methyl-p-tyrosine, a DA synthesis inhibitor. Additionally, the stimulated release of reserve pool DA, mobilized by cocaine (COC), was measured. Both efflux and stimulated release measurements were enhanced in slices from knockout mice, suggesting that these mice have greater reserve pool DA stores than wild-type and that these stores are effectively mobilized. Moreover, dopamine transporter (DAT) labeling data indicate that the difference in measured DA efflux was likely not caused by altered DAT protein expression. Additionally, slices from MsrA−/− and wild-type mice were equally responsive to increasing extracellular calcium concentrations, suggesting that potential differences in either calcium entry or intracellular calcium handling are not responsible for increased reserve pool DA release. Collectively, these results demonstrate that MsrA−/− knockout mice maintain a larger DA reserve pool than wild-type control mice, and that this pool is readily mobilized.

Research highlights▶Dopamine reserve pools were quantified in MsrA−/− mice and WT control mice. ▶MsrA−/− mice had an enhanced dopamine reserve pool compared to WT mice. ▶Reserve pool mobilization occurred over a longer time period in MsrA−/− mice. ▶Dopamine release was equally sensitive to extracellular Ca2+ in MsrA−/− and WT mice. ▶Study among first to measure reserve pool dopamine in an oxidative stress model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 177, 17 March 2011, Pages 223-229
نویسندگان
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