کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6278072 | 1295793 | 2009 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Zic1 levels regulate mossy fiber neuron position and axon laterality choice in the ventral brain stem
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کلمات کلیدی
PBSMCPeGFPEMSPFADCCPGNdeleted in colorectal cancer - در سرطان کولورکتال حذف شده استpostnatal day - روز پس از زایمانTranscription factor - عامل رونویسیmossy fiber - فیبر قارچPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCerebellum - مخچهMouse - موشparaformaldehyde - پارافرمالدهیدenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته است
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Zic1 levels regulate mossy fiber neuron position and axon laterality choice in the ventral brain stem Zic1 levels regulate mossy fiber neuron position and axon laterality choice in the ventral brain stem](/preview/png/6278072.png)
چکیده انگلیسی
Pontine gray neurons of the brain stem are a major source of mossy fiber (MF) afferents to granule cells of the cerebellum. Achieving this connectivity involves an early regionalization of pontine gray neuron cell bodies within the brainstem pontine nuclei, as well as establishing the proper ratio of crossed versus uncrossed MF projections to contralateral versus ipsilateral cerebellar territories. Here, we report expression of the transcription factor Zic1 in newly postmitotic pontine gray neurons and present functional experiments in embryonic and postnatal mice that implicate Zic1 levels as a key determinant of pontine neuron cell body position within the pons and axon laterality. Reducing Zic1 levels embryonically via in utero electroporation of short hairpin RNA interference (shRNAi) vectors shifted the postnatal distribution of pontine neurons from caudolateral to rostromedial territories; by contrast, increasing Zic1 levels resulted in the reciprocal shift, with electroporated cells redistributing caudolaterally. Associated with the latter was a change in axon laterality, with a greater proportion of marked projections now targeting the ipsilateral instead of contralateral cerebellum. Zic1 levels in pontine gray neurons, therefore, play an important role in the development of pontocerebellar circuitry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 162, Issue 3, 1 September 2009, Pages 560-573
Journal: Neuroscience - Volume 162, Issue 3, 1 September 2009, Pages 560-573
نویسندگان
H.J. DiPietrantonio, S.M. Dymecki,