کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6286266 | 1615300 | 2014 | 8 صفحه PDF | دانلود رایگان |
- In gilt PCG, overdose of testosterone reduces the total number of ovarian perikarya.
- A decrease in the set of cholinergic perikarya in testosterone-affected porcine PCG.
- Increased set of AR-positive ovarian perikarya in PCG after testosterone injections.
- Hyperandrogenism in pathological state may regulate ovary function by effect on PCG.
The influence of testosterone (T) overdose on the number and distribution of ovarian neurons in the paracervical ganglion (PCG) in pigs was examined. To identify the ovarian neurons, on day 3 of the estrous cycle, the ovaries of both the control and experimental gilts were injected with retrograde neuronal tracer Fast Blue. From next day to the expected day 20 of the second studied cycle, experimental gilts were injected with T, while control gilts received oil. The PCG was then collected and processed for double-labeling immunofluorescence. T injections increased the T (â¼3.5-fold) and estradiol-17β (â¼1.6-fold) levels in the peripheral blood, and reduced the following in the PCG: the total number of Fast Blue-positive neurons, the number of perikarya in the lateral part of the PCG, the numbers of VAChT+/SOM+, VAChT+/VIP+, VAChT+/nNOS+, VAChT+/VIPâ, VAChT+/DβHâ, VAChTâ/SOMâ, VAChTâ/VIPâ, VAChTâ/nNOSâ and VAChTâ/DβHâ perikarya, In the T-affected PCG, the populations of ovarian perikarya coded VAChTâ/SOM+, VAChTâ/VIP+ and VAChTâ/DβH+, and expressing androgen receptor were increased. After T treatment within the PCG dropped the density of nerve fibers expressing VAChT and/or SOM, VIP, DβH. Obtained data suggest that elevated androgen levels occurring during pathological processes may regulate ovary function(s) by affecting the PCG gonad-supplying neurons.
Journal: Neuroscience Research - Volume 83, June 2014, Pages 89-96