کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6287917 | 1300020 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An exo-1,3-β-glucanase GLU1 contributes to the virulence of the wheat tan spot pathogen Pyrenophora tritici-repentis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An exo-1,3-β-glucanase GLU1 contributes to the virulence of the wheat tan spot pathogen Pyrenophora tritici-repentis An exo-1,3-β-glucanase GLU1 contributes to the virulence of the wheat tan spot pathogen Pyrenophora tritici-repentis](/preview/png/6287917.png)
چکیده انگلیسی
Tan spot, caused by Pyrenophora tritici-repentis, is an important foliar disease of wheat. In the present study, a gene named glucanase gene (GLU1) encoding a putative exo-1,3-β-glucanase was cloned from a race five isolate of P. tritici-repentis. Transcription profile analysis of the GLU1 gene showed a carbon source control of the accumulation of transcript, which is strongly induced in minimal medium but depressed in rich medium. A time-course study of the infection process of the wild-type isolate on a susceptible wheat genotype revealed that the transcript level of GLU1 increased more than 8000-fold 8 h after inoculation. To study its potential function in pathogenicity, GLU1 was silenced via a sense and antisense mediated silencing mechanism. One transformant named C1 showed significantly reduced growth and sporulation relative to the wild-type. Cytological analysis of the infection revealed that C1 produced significantly lower numbers of germ tubes and appressoria than the wild-type strain on susceptible wheat leaves. This strain, as well as another two transformants, caused significantly less disease symptoms relative to the wild-type after inoculation onto a susceptible wheat genotype. These results indicate that GLU1 contributes to the development and virulence of P. tritici-repentis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Biology - Volume 117, Issue 10, October 2013, Pages 673-681
Journal: Fungal Biology - Volume 117, Issue 10, October 2013, Pages 673-681
نویسندگان
Heting Fu, Jie Feng, Reem Aboukhaddour, Tiesen Cao, Sheau-Fang Hwang, Stephen E. Strelkov,