کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6288488 | 1616249 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptomic and proteomic analysis of Oenococcus oeni PSU-1 response to ethanol shock
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Transcriptomic and proteomic analysis of Oenococcus oeni PSU-1 response to ethanol shock Transcriptomic and proteomic analysis of Oenococcus oeni PSU-1 response to ethanol shock](/preview/png/6288488.png)
چکیده انگلیسی
The correct development of malolactic fermentation depends on the capacity of Oenococcus oeni to survive under harsh wine conditions. The presence of ethanol is one of the most stressful factors affecting O. oeni performance. In this study, the effect of ethanol addition (12% vol/vol) on O. oeni PSU-1 has been evaluated using a transcriptomic and proteomic approach. Transcriptomic analysis revealed that the main functional categories of the genes affected by ethanol were metabolite transport and cell wall and membrane biogenesis. It was also observed that some genes were over-expressed in response to ethanol stress (for example, the heat shock protein Hsp20 and a dipeptidase). Proteomic analysis showed that several proteins are affected by the presence of ethanol. Functions related to protein synthesis and stability are the main target of ethanol damage. In some cases the decrease in protein concentration could be due to the relocation of cytosolic proteins in the membrane, as a protective mechanism. The omic approach used to study the response of O. oeni to ethanol highlights the importance of the cell membrane in the global stress response and opens the door to future studies on this issue.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 51, October 2015, Pages 87-95
Journal: Food Microbiology - Volume 51, October 2015, Pages 87-95
نویسندگان
Nair OlguÃn, Marie Champomier-Vergès, Patricia Anglade, Fabienne Baraige, Ricardo Cordero-Otero, Albert Bordons, Monique Zagorec, Cristina Reguant,