Identification of Lactobacillus curvatus TMW 1.624 dextransucrase and comparative characterization with Lactobacillus reuteri TMW 1.106 and Lactobacillus animalis TMW 1.971 dextransucrases

► Exopolysaccharide (EPS) production through Lactobacillus curvatus, Lactobacillus reuteri and Lactobacillus animalis. ► Comparison of genetic background and enzymatic properties. ► Determination of optimal pH and temperature for glycosyltransferase activity. ► Stimulation of glucosyltransferases through various EPSs and metal ions. ► Structural comparison of EPSs produced by enzymes versus wildtype cells.