The combination of CRISPR-MVLST and PFGE provides increased discriminatory power for differentiating human clinical isolates of Salmonella enterica subsp. enterica serovar Enteritidis

► CRISPR-MVLST identifies several unique Salmonella Enteritidis Sequence Types. ► Combination of CRISPR-MVLST and PFGE provides a high discriminatory power. ► Subtle differences among different CRISPR alleles, suggesting vertical evolution.