کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6293190 | 1617133 | 2016 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A review of toxicity testing protocols and endpoints with Artemia spp.
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
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چکیده انگلیسی
Artemia spp. is an historically popular biological model still requiring an official internationally based standardization. Several endpoints are currently available. Short-term acute endpoints include biomarker (acetylcholinesterase; heat stress proteins; lipid peroxidation; thiobarbituric acid reactive substances; thioredoxin reductase; glutathione-peroxidase; glutathione S-transferase; glutathione reductase; aldehyde dehydrogenase; and adenylpyrophosphatase and Fluotox), hatching (dry biomass, morphological disorders and size), behavioral (swimming speed and path length), teratogenicity (growth), and immobilization (meaning mortality after 5-30Â s observation). Long-term chronic tests focus on growth, reproduction and survival or mortality after 7-28 d exposure from larval to adulthood stage. We analyzed each test looking at its endpoint, toxicant and experimental design including replicates, exposure time, number of exposed cysts or organisms and their relative life stage, exposure conditions during hatching and testing (salinity, pH, light intensity, aeration dilution media, and food supply), type of testing chambers, and quality assurance and quality control criteria. Similarities and differences between the identified approaches were highlighted. Results evidenced that hatching 24Â h short-term and 14 d long-term mortality are the most promising Artemia spp. protocols that should go forward with international standardization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecological Indicators - Volume 69, October 2016, Pages 35-49
Journal: Ecological Indicators - Volume 69, October 2016, Pages 35-49
نویسندگان
G. Libralato, E. Prato, L. Migliore, A.M. Cicero, L. Manfra,